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Spirent KB Article
Doc ID: FAQ17000
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iTest: Getting error "COM1: SerOpenPort failed: Port is in use" while connecting to the Serial port using iTest Serial session.
Close the iTest and open it from the command prompt with "-clean" option.
Ex: C:\Program Files\Spirent Communications\iTest 5.1>iTest.exe -clean
Start a Serial session and see if you are able to connect to the port.
Product : iTest Enterprise